Characterization of RD-114 virus isolated from a commercial canine vaccine manufactured using CRFK cells.
نویسندگان
چکیده
Recently, we found that several commercial pet vaccines were contaminated with an infectious endogenous retrovirus, RD-114-related virus. Here, we determined the entire nucleotide sequences of RD-114-related viruses isolated from CRFK cells and a vaccine manufactured using CRFK cells. These RD-114-related viruses were nearly identical to the authentic RD-114 virus.
منابع مشابه
A New Approach to Establish a Cell Line with Reduced Risk of Endogenous Retroviruses
Endogenous retroviruses (ERVs) are integrated as DNA proviruses in the genomes of all mammalian species. Several ERVs are replication-competent and produced as fully infectious viruses from host cell. Thus, live-attenuated vaccines and biological substances have been prepared using the cell lines which may produce ERV. Indeed, we recently reported that several commercial live-attenuated vaccine...
متن کاملIsolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies
Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was ampl...
متن کاملEndogenous feline retrovirus RD-114 does not elicit neutralizing antibodies in dogs.
At least two retroviruses, endogenous feline leukemia virus (FeLV) and RD-114 virus, are assumed to be integrated in the genome of domestic cats (1). Several feline cell lines have been shown to produce RD-114-related viruses (2). In Japan, feline cell lines are widely used to produce vaccines for dogs, particularly against canine parvovirus. The level of infectious RD-114 virus found in canine...
متن کاملIsolation, Characterization and Standardization of New Infectious Bursal Disease Virus for Development of a Live Vaccine
Background and Aims: Infectious bursal disease (IBD) is an acute contagious viral disease of birds worldwide. The causative virus induces a persistent immune suppression following destroy B lymphocytes precursors in bursal lymphoid follicles. Vaccination is the main strategy for prevention of the disease in commercial poultry industry. Materials and Methods: To produce a live vaccine against th...
متن کاملCell receptors for baboon endogenous virus recognized by monoclonal antibodies.
Hybridomas from mice immunized with baboon endogenous virus (BaEV) from A204(M7) cells produced several antiviral monoclonal antibodies and, in addition, antibodies D-12 and E-4, which appeared to be virus specific because they reacted with BaEV but not with Mason-Pfizer virus or RD-114 virus. However, they also bound to human virus-free cells, and they did not recognize BaEV from bat or canine...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 48 9 شماره
صفحات -
تاریخ انتشار 2010